DNA HAVING GENETIC INFORMATION OF POLYPEPTIDE HAVING ASCORBATE OXIDASE ACTIVITY ORIGINATED FROM CUCURBITA-MOSCHATA AND USE THEREOF

摘要

PURPOSE:To produce on ascorbate oxidase by culturing a host cell transformed with a DNA-containing recombined DNA coding a polypeptide having an ascorbate oxidase activity originated from Cucubita.moschata. CONSTITUTION:A host cell such as a bacterium cell, e.g. Escherichia coli cell or Bacillus.subtillus cell, yeast, plant cell or animal cell is transformed with a recombined DNA containing DNA which is a basic sequence coding a Cucurbita.moschata-originated polypeptide having an ascorbate oxidase activity. The transformed host cell is cultured in a medium to produce the Cucurbita.moschate-originated polypeptide having the ascorbate oxidase activity. The cultured cells are subjected to a lysozyme treatment, a ultrasonic crushing treatment, etc., to isolate the Cucurbita.moschata-originated polypeptide having the ascorbate oxidase activity.