PURPOSE:To obtain an immobilized lipase capable of excellently hydrolyzing fats and oils by introducing a hydrophobic group to part of amino group and hydroxyl group of chitosan to form a flock and then adsorbing lipase in the flock. CONSTITUTION:A hydrophobic group such as alkyl group or benzyl group is introduced to part of amino group and hydroxyl group of chitosan, a flock is formed from the chitosan and then lipase is adsorbed on the flock to give an immobilized lipase. Fats and oils can be hydrolyzed by using the lipase. As a method of hydrolyzing the fats and oils, a method of dispersing the immobilized lipase into fats and oils and hydrolyzing the fats and oils or a method of passing fats and oils through a column packed with the immobilized lipase and hydrolyzing the fats and oils may be cited. Since the immobilized lipase is different from gel and consists of the flock having both a hydrophilic group and a hydrophilic group, both fats and oils and water can be simply permeated into the immobilized lipase.
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