ANTI-HUMAN MYOCARDIAL PROTEIN C MONOCLONAL ANTIBODY AND HYBRIDOMA PRODUCING THE SAME ANTIBODY

摘要

PURPOSE:To produce an anti-human myocardial protein C monoclonal antibody by proliferating hybridoma obtained by fusing an antibody-producing cell from an animal immunized with a human myocardial C protein as an antigen and a myeloma cell. CONSTITUTION:An antibody-producing cell collected from an animal immunized with a human myocardial protein C and a myeloma cell are subjected to cell fusion to obtain a hybridoma. Said hybridoma is proliferated in an animal abdominal cavity or in a culturing solution and an anti-human myocardial protein C monoclonal antibody is collected from abdominal ascites or a cultured solution. Said monoclonal antibody is especially usable as a diagnostic containing an anti-human myocardial protein C monoclonal antibody whose labelled substance with enzyme, radioisotope or a fluorescent compound can specifically recognize said human myocardial protein C.