GENE FRAGMENT CODING STATIONARY REGION OF CANINE IMMUNOGLOBULIN KAPPA CHAIN AND MOUSE X DOG CHIMERA ANTIBODY

摘要

NEW MATERIAL:A gene fragment having a DNA sequence coding a stationary region of a canine immunoglobulin kappa chain expressed by formula, etc. USE:Production of mouse X dog chimera antibody for the diagnosis, remedy and prevention of viral diseases of dog. PREPARATION:The objective gene fragment coding the stationary region of canine immunoglobulin kappa chain can be produced e.g. by separating chromosome DNA from canine hepatocyte by conventional process, cutting the DNA with restriction enzyme, developing the fragments on 0.7% agarose gel by electropho resis, transferring to a nitrocellulose membrane filter, subjecting to Southern hybridization with [32P]-labeled DNA probe containing human Ckappa region, separat ing a fragment forming a single band of 5 kb, bonding the fragment to a vector to prepare a kappa-chain gene library and screening the library by conventional method.