PREVENTIVE VACCINE FOR BACTERIAL DISEASE OF PRAWN AND PRODUCTION THEREOF

摘要

PURPOSE:To provide the title vaccine for bacterial diseases of prawn such as vibriosis, containing as active ingredient, deactivated vibrio penaeus. CONSTITUTION:The objective vaccine (in the form of a solution) containing deactivated vibrio penaeus by making a shaking culture at 25 deg.C for ca.24hr in a 50% seawater bouillon medium of vibrio penaeus, a new strain of Vibrio bacteria separated from sick prawn and by either adding ca.0.5% of formalin to the culture solution or heating treatment of the solution to kill the bacteria. The number of the bacteria in the present vaccine is pref. ca.109 cells/ml. The present vaccine can be used by dilution with seawater by a factor of 10 to 1000 and either spraying on prawn or immersing the prawn in the diluted solution; alternatively, it is usable in the following manner: after killing bacteria, a centrifugation is made to remove the culture solution followed by washing the microbial cells with 50% seawater and then carrying out a centrifugation to remove the seawater to obtain the microbial cells, and 0.05-1.0wt.% of the cells is mixed with a feed for prawn larvae, the resulting feed is put to oral administration.