PHOSPHORAMIDITE COMPOUND AND METHOD FOR SYNTHESIZING OLIGORIBONUCLEOTIDE IN SOLID PHASE USING THE SAME

摘要

NEW MATERIAL:A compound expressed by formula I (B' is nucleic acid base residue; R1 is protecting group of OH group; R2 is protecting group of phosphoric acid; X is group reactive with OH groups of sugar parts in ribonucleosides or other ribonucleotides). EXAMPLE:5'-O-(Dimethoxytrityl)-2'-O-(2-chloroethoxyethyl)-N6-(benzoyl) adenosine-3'-O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite. USE:A synthetic material for oligoribonucleotides in a high yield and purity. PREPARATION:A ribonucleoside expressed by formula II is reacted with 1,3- dichloro-1,1,3,3-tetraisopropyldisiloxane, etc., to provide a compound expressed by formula III, which is then reacted with an amiditing agent (e.g. chloro-2- cyanoethoxy-N,N-diisopropylaminophosphane) expressed by formula IV.