METHOD FOR OBTAINING HIGHLY GLYCYRRHIZIN-PRODUCING STRAIN OF GLYCYRRHIZA GLABRA L. VAR ROOT STEM CELL

摘要

PURPOSE:To enable to efficiently produce glycyrrhizin by transplanting the root stem cells of Glycyrrhiza glabra L. var from a plant tissue culture medium to any other medium changed in the concentration of a plant growth hormone to give a chemical stress to the cells. CONSTITUTION:An auxin such as 2,4-D, a cytokinin such as benzyladenine, etc., are added to a medium such as a MS medium containing a large amount of an inorganic salt such as NH4NO3, a fine amount of inorganic salt such as FeSO4, organic nutrients such as vitamins and a carbon source such as sucrose, and the root stem cell of Glycyrrhiza glabra L. var is cultured in the prepared medium. On the culture, the cells are transferred from the medium containing the hormones into a medium not containing the hormones during the culturing process, or from the medium having a hormore concentration of approximately 0.5-20ppm into a medium having a hormone concentration of approximately 0.01-0.5ppm to give a chemical stress to the Glycyrrhiza glabra L. var. The difference between the hormone concentrations is required to be =0.5ppm. The secondary metabolic system of the plant is stimulated by the chemical stress to accelerate the production of glycyrrhizin.