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PROCESS FOR CULTIVATING NITRIFICATING MICROORGANISMA FIRST OF ALL ON SOLIDE MEDIUM AND FOR IMPROVING RECOGNIZING THEM
PROCESS FOR CULTIVATING NITRIFICATING MICROORGANISMA FIRST OF ALL ON SOLIDE MEDIUM AND FOR IMPROVING RECOGNIZING THEM
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机译:首先在固体培养基上培养硝化微生物并提高其识别率的过程
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Culturing atmospheric nitrogen-fixing bacterial primarily on solid substrates to facilitate detection. - Substrates are prepd. in two stages. The base substrate contains (all wt.%): 1) Na2HPO4.12H2O (1.11-1.56); 2) KH2PO4 (0.13-0.18); 3) Dist.H2O. (92.8-96.19); 4) ionic agar (1.45-2.01); and is sterilised pref. at 121 deg.C. - Another soln. is prepd. separately, contg. 5) MgSO4.7H2O (0.011-0.033); 6) CaCl2.2H2O (0.001-0.002); 7) FeCl3 (0.0001-0.001); 8) Na2MoO4.2H2O (0.001-0.0003) and sterilised similarly to the base substrate and then; 9) D-Mannitol sol. (1.11-3.35) sterilised by filtration. Following this, components 9,6,7,8 and 5 (pref. in this order) are added to the base substrate (pref. cooled to 50 deg.C); under thorough agitation and sterile concns. - The solid minimal nutrient prepd. has a pH of 7.2-7.45 pref. 7.35. This minimal, nitrogen-free substrate may be prepd. without ionic agar
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