MONOCLONAL ANTIBODY REACTIVE TO A UNIQUE ANTIGEN WIDELY PRESENT ON VARIOUS HUMAN LEUKEMIA AND LYMPHOMA CELLS AND METHOD OF USING SAME FOR DIAGNOSIS AND TREATMENT

摘要

A novel hybrid cell line, designated T6-1G9, for production of monoclonal antibodies specific for a unique cell surface antigen, GP20, associated with a wide variety of human lymphomas and leukemias. Hybridoma T6-1G9 was generated by fusing mouse myeloma cells with spleen cells from a BALB/c mouse that was immunized with a human leukemia antigen preparation isolated from the cell membranes of acute lymphoblastic leukemia cells. This invention also provides a method for producing the new monoclonal antibody designated SN7 and to diagnostic procedures using SN7 to detect various leukemias and lymphomas. Also disclosed are methods of using SN7 or reactive fragments of SN7 for the treatment of leukemia-lymphoma patients.