METHOD OF REGENERATING RICE PLANT FROM PROTOPLASTS

摘要

- 23 -ABSTRACT OF THE DISCLOSUREMethod of generating a complete plant of rice from aprotoplast thereof. To prepare the protoplast, a callus isderived from an embryo, scutellum, or radicle of a seed ofrice. The callus is then cultured in a liquid medium toform a small cell cluster which readily releases aprotoplast. The cell cluster is then treated with aprotoplast-releasing enzymatic solution to obtain aprotoplast. The protoplast is then cultured to form acallus, and the callus is then cultured in adifferentiation medium to regenerate a whole plant ofrice.