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METHOD OF ASSAYING OR ANALYZING SUBTYPES OF HUMAN LEUKOCYTE INTERFERONS OR THEIR ANTIBODIES, AND ANTIBODIES TO BE USED THEREFOR
METHOD OF ASSAYING OR ANALYZING SUBTYPES OF HUMAN LEUKOCYTE INTERFERONS OR THEIR ANTIBODIES, AND ANTIBODIES TO BE USED THEREFOR
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机译:评估或分析人类白细胞干扰素或其抗体亚型的方法,以及将要使用的抗体
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摘要
of the disclosure: Antiserum which can recognize all subtypes of human leukocyte interferon is prepared from the blood of an animal immunized with partially purified human leukocyte interferon obtained from a culture of human leukocyte stimulated with Sendai virus. The antiserum is added to a column whereon concentrated culture broth of human leukocyte has been immobilized to adsorb impurities, the effluent is added to a column whereon partially purified human leukocyte interferon has been immobilized to adsorb anti-human leukocyte interferon antibody and then the antibody is eluted from the column. The antibody thus obtained recognizes all subtypes of human leukocyte interferon and can be separated by a chromatography to each monoclonal antibody which recognizes a single subtype. Employing these antibodies, the subtypes of human leukocyte interferon or their antibodies in a sample can be analized or assayed.
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