Method for the purification of the HBc antigen and method for the measurement of HBc antibodies by using said purified HBc antigen

摘要

Disclosed is a method for the purification of HBC antigen, particularly HBc antigen produced by recombinant organisms constructed by means of DNA recombination techniques, which comprises subjecting a solution containing HBc antigen to acid-treatment, wherein the solution containing HBc antigen is acidified to a pH range of not higher than 6 by adding an acid, removing the resulting precipitates of lipids and contaminant proteins, and then subjecting the acid-treated solution containing HBc antigen to an ion exchange chromatography with an anion exchanger. Furthermore, a method for measuring HBc antibodies is described in which said purified HBc antigen is used in a passive hemagglutination method, EIA method or RIA method.