Amplification of hybridization signals by employing complementary DNA strands

摘要

A method for assaying sample for a target ligand includes the step of contacting sample with reagent from a kit including reagent probe, amplifier strand and label strand. The reagent probe is capable of binding to the target ligand and includes a polynucleotide tail portion. The amplifier strand and label strand bind to the tail portion to form a polynucleotide network. The label strand includes a label moiety capable of detection.