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Antibodies to human cancer nucleolar antigen pl20, corresponding cell lines, and their preparation and use in the detection of human cancer cells
Antibodies to human cancer nucleolar antigen pl20, corresponding cell lines, and their preparation and use in the detection of human cancer cells
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机译:人癌核仁抗原p120抗体,相应的细胞系及其制备和用于检测人癌细胞
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Tumor nucleoli were treated with polyclonal antisera to normal human tissue nucleoli to block determinants common to tumor and normal tissue nucleoli. Immunization of mice with these immune-complexes resulted in the development of a monoclonal antibody (FB2) to a novel nucleolar proliferation associated antigen which has a molecular weight of about 120 kD and a pI of about 4.5. By indirect immunofluorescence, antibody FB2 produced bright nucleolar staining in a variety of malignant tumors, including cancers of the breast, liver, gastrointestinal tract, genitourinary tract, blood, lung and brain. Specific nucleolar immunofluorescence was not detectable in most normal tissues, although it was weakly detectable in some proliferating nonmalignant tissues including spermatogonia of the tests, ductal regions of hypertrophied prostate, and PHA stimulated lymphocytes. Disclosed are characterizations and methods for isolating nucleolar antigen p120, characterizations and methods for producing antibodies including monoclonal antibody having binding specificity to nucleolar antigen p120, and diagnostic procedures and kits useful for detecting human cancer cells.
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