Detection of influenza-A-virus by polymerase chain reaction following the reverse transcription of a domain of the viral hemagglutinine gene

摘要

The sensitivity of detection of influenza A virus has been considerably increased by carrying out a polymerase chain reaction (PCR) using oligonucleotide sequences (amplimers) from the reasonably conserved region of the viral haemagglutinin gene after previous reverse transcription (RT). The reverse transcription and the PCR are in this case insensitive to single mutations by base exchange unless the 3' end of the amplimers is affected.