Method for preparing Death (64,65) -Proinsulin

摘要

This application describes a process for producing des(64,65)- human proinsulin from human proinsulin by treating the latter with trypsin and carboxypeptidase B. The process is conducted by applying any or all of the following parameters: (1) use of an alkylated or acylated trypsin; (2) a temperature of from about 0 C. to about 10 C.; (3) a pH of from about 8 to about 10; and (4) use of a buffer selected form the group consisting of ammonium bicarbonate and glycine.