RECOMBINANT PLASMIDE DNA P1EM3 ENCODING SYNTHESIS OF B-SUBUNIT OF CHOLERA TOXIN, METHOD OF ITS CONSTRUCTING, AND STRAIN OF VIBRIO CHOLERAE BACTERIA PRODUCING B-SUBUNIT OF CHOLERA TOXIN

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 biotechnology, medicine and veterinary medicine. purpose u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 more productive u043fu0440u043eu0434u0443u0446u0435u043du0442u0430 in u0441u0443u0431u044cu0435u0434u0438u043du0438u0446u044b v toxin. designed to be re u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u043au043eu043du044cu044eu0433u0430u0442u0438u0432u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u0430 u04401u0435u043cu0437 by u043eu0431u044au0435u0434u0438u043du0435u043du0438u00a0 by 151 - u0437u043bu0435u043cu0435u043du0442u0430 imposed by the u043au043eu043du044cu044eu0433u0430u0442u0438u0432u043du043eu0439 plasmid plEMI,. all plasmids plEMI, u043au043eu043du044cu044eu0433u0430u0442u0438u0432u043du043eu0439 u0440u043eu044538 derived plasmids.the labeled mini u0442u0440u0430u043du0441u043fu043eu0437u043eu043du043eu043c gene u0440u0435u0437u0438u0441u0442u044du043du0442u043du043eu0441u0442u0438 to t and u043du0430u043cu0438u0446u0438u043du0443 (ct and pct 27. u043fu0440u04411 1u0432u043eu0434u043du043eu0439 pBR 322. the load in its u0441u043eu0441u0433u0430u043fu0435 vet cloned gene and marker u0440u0435u0437u0438u0441u0442u0435u043du0442u043du0435u0441u0442u0438 to u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0443 (tc). by u043au043eu043du044cu044e1u0430u0446u0438u0438 u043fu043bu0430u0437u043cu0438u0434u0430 u04401u0435u043cu0437 moved from strains of e. coli to 12 in a strain of v. cholerae u0441197 - 01 group. among the selected u0442u0440u0430u043du0441u043au043eu043du044cu044eu0433u0430u043du0442u043eu0432 b1 -.| de len u044cu0438u0442u0430u043cu043c u043au043c93, lost a part of our u043au043eu043du044cu044eu0433u0430u0442u0438u0432u043du043eu0439 u043au043eu0438u043du0442u0435u0433u0440u0430u0442u0430. yu c separate.u0438u0439u0441u00a0 increased resistance to u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0443, stable persistence of this characteristic, and capable of efficiently producing the u0441u04436u044cu0435u0434u0438u043du0438u0446u0443 u0445u043eu043bu0435u0440u043du043e the toxin in the amount of 10 - 11 mg / l u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 environment.the ability to u0440u043eu0434u0443u043au0446u0438u0438 (u043bu0442u0430u043cu043cu0430 u043au043c93 u044du043au0437u043eu0433 - in - u0441u0443u0431u044cu0435u0434u0438u043du0438u0446u044b v u0442u043eu043au0433u0438 at defined using three methods: the immune reaction of hemolysis in a u043fu0430u0441u0441u0438u0432u043du043eu044e the cac, immune enzyme method, elisa, u0442u0438u0442u0440u0430u0446u0438u0438 drug in the skin sample for craig. and u0442u0430u043au0445u0435 in production u0443u0441u043bu043eu0432u0438u00a0u0445 in cultivation of strain in the reactor.it is shown that the strain u043au043c93 not u043fu0430u0442u043eu0433u0435u043du0435u043d u0434u043bu00a0 u043au0440u043eu043bu044cu0447u0430u0442 - suckers at the dose of 10 - 10 microbial cells and is not u043du0430u043au043eu043fu043bu0435u043du0438u00a0 fluid in the small intestine of adult rabbit 3. u043f.u0444 - lu. u0441u00a0 of eat of yu yu