HYBRID ANIMAL CULTURE CELL STRAIN MUSCULUS L PRODUCING MONOCLONAL ANTIBODIES TO MEMBRANE ANTIGEN MYCOPLASMA ANGININI

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 biotechnology and can be used u0434u043bu00a0 diagnosis u043cu0438u043au043eu043fu043bu0430u0437u043c - u043au043eu043du0442u0430u043cu0438u043du0430u043du0442u043eu0432 in cell cultures. the strain produced u0441u043bu0438u00a0u043du0438u0435u043c mouse sp2 / 0 myeloma cells with spleen cells of mice BALB ag / c immunized mebran M.arglnini antigen.the cells grow in the environment u0434u043cu044du043c with addition of 10% serum u0444u0435u0442u0430u043bu044cu043du043eu0439 cattle, 1 mm u043fu0438u0440u0443u0432u0430u0442u0430 u043du0430u0442u0440u0438u00a0, 2% u0433u043bu0443u0442u0430u043cu0438u043du0430, 100 micrograms / ml u0441u0442u0440u0435u043fu0442u043eu043cu0438u0446u0438u043du0430 and 100 iu / ml u043fu0435u043du0438u0446 u0438u043bu043bu0438u043du0430, frequency u043fu0430u0441u0441u0438u0440u043eu0432u0430u043du0438u00a0 1 - 2 days, the index screening of v2 and v3. the cells produce my at specific to u0430u043du0442u0438u0433u0435u043du0443 Mycoplasma arginini, titer of my at in u0438u043cu043cu0443u043du043eu0444u0435u0440u043cu0435u043du0442u043du043eu043c test u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 1 - 128 - 1 256