process for production of alkaline serine protease from ammoniumlimitierte fermentation of bacillus strains

摘要

for the extraction of subtilisin are bacteria of the genus bacillus in a fermentor cultivated on synthetic medium, with a first anwachsphase with nh4 - initial concentration of 0.5 to 150 mmin particular, 1 to 50 mm and nachdosierung c source for the maintenance of their concentration in the range of 100 mm to 1 m to the fall in the ammoniumkonzentration under an n - limiting value in the culture of between 0.05 and 2 mm, in particular, 0.1 and 0.3 mm is used.according to the n - limiting value of ammoniumkonzentration maintained and the content of the c - source between 10 mm and 2 m is set to the end of the production phase, which is formed subtilisin isolated. as c source, in particular, glycerol, acetate, or used in organic chemistry. preferably, the biomassekonzentration in medium by nachfu00fcttern c source increases.a partial pressure of oxygen on the culture of 11.5 pa is appropriate. as proteaseproduzent serves in particular bacillus licheniformis dsm 1969.