Gene comparison by restriction length analysis - using fluorescent labels, used to diagnose genetic defects

摘要

Comparison of genes is effected by (a) cleaving two or more genes with the same enzyme; (b) labelling the DNA fragments from each gene with a different fluorescent label; (c) mixing all the labelled fragments; (d) further cleaving the fragments in the mixt. with an enzyme; (e) separating the fragments; and (f) optically detecting any differences in the pattern of sepd. fragments. Pref., step (e) is effected by one- or multi-dimensional gel electrophoresis. Step (f) is effected using a real-time fluorescence detector or a one- or two-dimensional gel reader, such that light emitted from the same site at the same time is analysed according to wavelength. USE/ADVANTAGE - The process may be used to diagnose genetic defects by comparing genes from a test subject with the corresp. genes from a normal subject, or to distinguish between the genes of different individuals. The process allows genes to be distinguished with great efficiency and precision, even when they have a very similar nucleotide sequence.