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PROCESS FOR PRODUCING A POLYPEPTIDE HAVING DETERMINED SELECTED PROPERTIES, IN A HOST ORGANISM TRANSFORMED BY RECOMBINANT DNA CONTAINING NUCLEIC SEQUENCES ENCODING THE POLYPEPTIDE AND A BONE AFFIN PROTEIN
PROCESS FOR PRODUCING A POLYPEPTIDE HAVING DETERMINED SELECTED PROPERTIES, IN A HOST ORGANISM TRANSFORMED BY RECOMBINANT DNA CONTAINING NUCLEIC SEQUENCES ENCODING THE POLYPEPTIDE AND A BONE AFFIN PROTEIN
The invention relates to a method for producing, optionally exporting, and purifying a predetermined polypeptide. It comprises the step of causing the expression in a host organism, particularly a bacteria E. coli, of a recombinant nucleic acid coding for a hybride polypeptide containing a sequence coding for the protein MalE or MalX, and a sequence coding for the polypeptide searched for, the recovery of the hybride polypeptide by akin chromatography on an amylose column and the recovery of the polypeptide by elution by means of a maltose solution. The invention relates also to applications of the hybride polypeptides thus obtained, particularly in the field of production of vaccine compositions by using MalE or MalX proteins as vector polypeptides for the presentation of epitopes.
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