This research and preparation of probes in the sequence, the oligonucleotides and probes obtained and method of detection of the pestivirus

摘要

In the process of searching for and for the preparation of probes common and / or specific of the types of sequence, it amplifies an area of about 110 to 115 bases located towards the end 5 ′ on the genome of a non-encoding sequence in the region corresponding to the region situated between the base 200 and 310 of the genome of the virus bvd osloss with the aid of a convergent primers hybridizing substantially at the level of the ends of this zone not coding sequence the zone is amplified and, by comparison with the similar zones already been sequenced other varieties of sequence, it is desired, the oligonucleotide sequences homologous and / or specific probes.