Method of identification and preparation of probes for pestiviruses, oligonucleotides and probes thus obtained and a method of detection of pestiviruses

摘要

In the method of identification and preparation of general and/or specific probes for varieties of Pestivirus, a length of approximately 110 to 115 bases, towards the 5' end on the Pestivirus genome in the non- coding region corresponding to the region situated between bases 200 and 310 of the genome of the BVD Osloss virus, is amplified by means of converging primers in a polymerase chain reactions, the amplified length is sequenced and, by comparison with similar previously sequenced lengths of other varieties of Pestivirus, the homologous and/or specific oligonucleotidic sequences of the probes are identified.