Method for identifying or shielding functional sites or epitopes of proteins that enter the exocytotic pathway of eukaryotic cells, the mutant proteins so produced and genes encoding said mutant proteins

摘要

The present invention relates to a method for identifying or shielding functional sites or epitopes of proteins that enter the exocytotic pathway of eukaryotic cells (transportable proteins) by the addition of supernumerary N-linked oligosaccharide side chains at chosen sites on the surface thereof using oligonucleotide mutagenesis. The present invention also relates to mutant transportable proteins having supernumerary N- linked oligosaccharide side chains which shield functional sites or epitopes; and genes which encode the same.