PRODUCTION OF PURIFIED RNA-BINDING PROTEIN AND METHOD FOR MEASURING ANTI-RNA-BINDING PROTEIN ANTIBODY

摘要

PURPOSE:To specifically and efficiently purify the subject protein useful as an antigenic reagent for autoantibody tests by extracting an RNA-binding protein from an animal tissue, etc., and subjecting the resultant RNA-binding protein-containing extract solution to special affinity gel chromatography. CONSTITUTION:An RNA-binding protein is initially extracted from an animal tissue, cultured cell or its processed substance and the resultant extract is then brought into contact with an affinity gel comprising a support containing polyuridylic acid polymer (e.g. polyuridylic acid-agarose) as a ligand under an environment of a salt-containing solution (preferably a 0.1M magnesium chloride solution). The RNA-binding protein adsorbed on the aforementioned affinity gel is eluted by a method (e.g. a gradient method) for increasing the salt concentration in the solution containing the aforementioned support suspended therein. Furthermore, the RNA-binding protein is preferably extracted by using an extracting buffer solution prepared by adding an inhibitor for proteolytic enzymes thereto at a physiological salt concentration.