PRODUCTION OF PURIFIED RNA-BINDING PROTEIN AND METHOD FOR MEASURING ANTI-RNA-BINDING PROTEIN ANTIBODY

摘要

PURPOSE:To specifically and efficiently fractionate the subject protein useful as an antigenic reagent, etc., for autoantibody tests from other proteins or ingredients by extracting an RNA-binding protein from an animal tissue, etc., and subjecting the resultant RNA-binding protein-containing extract to specific treatment. CONSTITUTION:An RNA-binding protein is initially extracted from an animal tissue, cultured cell or its processed substance and bivalent metallic ions (preferably Zn2+) are added to the resultant extract to agglutinate and deposit the RNA-binding protein. The agglutinated and deposited RNA-binding protein is then separated from other soluble proteins and a chelating agent (preferably an ethylenediaminetetraacetate) is subsequently added to redissolve the separated RNA-binding protein. The RNA-binding protein is preferably extracted by using an extracting buffer solution prepared by adding an inhibitor for proteolytic enzymes thereto twice at a physiological salt concentration. Furthermore, zinc chloride is preferably used for adding the bivalent metallic ions.