PREVENTION OF COLOR DEVELOPMENT OF COLOR REAGENT FOR PEROXIDASE DURING STORAGE AND COLOR REAGENT FOR PEROXIDASE UTILIZING THE SAME METHOD

摘要

PURPOSE:To improve shelf life by adding an anti-oxidizing agent to a solution of a color reagent for peroxidase, etc., and controlling the scattering of blank value. CONSTITUTION:A color reagent for peroxidase, e.g. a phenylenediamine, a benzidine, a biphenyldiamine, a diaminofluoreyne, an anisidine or 2, 2'-azino- bis-(3-ethylbenziazoline-6-solfonic acid) diammonium (ABTS) is dissolved in water to prepare a reagent solution (A). An anti-oxidizing agent (B) such as disodium calcium ethylene-diaminetetraacetate, erythorbic acid, guaiacum, isopropyl citrate or ascorbic acid is then added to the resultant (A) solution to prevent color development of the (A) solution during storage. As necessary, the (A) solution is blended with the component (B), thus producing the objective color reagent for peroxidase capable of storing without color development.