DNA IMMOBILIZED MICROSPHERE AND PURIFICATION OF DNA TRANSCRIPTION CONTROL FACTOR USING THE SAME MICROSPHERE

摘要

PURPOSE:To obtain DNA immobilized microspheres capable of being purified from a specific protein readily and in high yield, comprising a specific DNA chain bonded to a carrier composed of a resin containing epoxy group on the surface by covalent bond to subject the epoxy group to ring opening. CONSTITUTION:DNA immobilized microspheres comprising a DNA chain having a base sequence to specifically bond a specific protein, bonded to a carrier composed of a resin containing epoxy group on the surface by subjecting the epoxy group to ring opening and not adsorbing protein except the base sequence part. An epoxy group-containing (meth)acrylic ester (co)polymer may be cited as the resin to form the carrier surface and polyglycidyl methacrylate prepared by soap-free emulsion polymerization using especially a water-soluble azo-based polymerizing agent is preferable. A DNA transcription control factor may be cited as the protein to be purified by using DNA immobilized microspheres.