PRODUCTION OF MUTANT HAVING GENETIC MARKER AND DIFFERING IN GENETIC NATURE FROM PARENTAL STRAIN

摘要

PURPOSE:To obtain the title mutant for breeding, etc., by mutual mating of siitake mushroom mycelia with (respective) genetic marker(s) followed by culture of the resulting binuclear mycelia and by separating and incubating basidiospores from the resultant basidiocarps to effect reducing the risk of the death of genetic marker strain. CONSTITUTION:Mononuclear mycelia of siitake mushroom is either made into protoplast or subjected to a mixer together with an appropriate amount of sterilized water into mycelia pieces each made up of one to several cells. The product is then irradiated with ultraviolet rays or treated with a chemical into genetic marker strain. Thence, mutual mating or cell fusion is made between two kinds of siitake mushroom mycelia with said genetic markers respectively or such two kinds of mycelia with one of them having said genetic marker, and the resulting binuclear mycelia is incubated using the fungal bed of a mushroom medium such as material wood, and basidiospores are separated from the resulting basidiocarps and incubated, thus obtaining the objective mutant.