Production manner null of the adjustment protein (NEF) of HIV-I which uses human cell system

摘要

In a process for obtaining a regulation protein (nef) for HIV-1 using human astrocytes and/or glia cell lines, the cell line is infected with HIV-1 by coculturing, the infected cells are cloned and, if necessary, clones producing large quantities of nef are selected by sub-cloning. A particularly well suited cell line is the human cell line TH4-7-5 deposited with the Collection Nationale de Cultures de Microorganismes (CNCM) Paris, under the number I-861.