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PROCESS FOR PRODUCING DEACETOXY-CEFALOSPORIN-C-SYNTHETASE AND PROTEIN HAVING DEACETYL-CEFALOSPORIN-C-SYNTHETASE ACTIVITY, HOST CELLS CAPABLE OF EXPRESSING PROTEIN, AS WELL AS CEFALOSPORIN
PROCESS FOR PRODUCING DEACETOXY-CEFALOSPORIN-C-SYNTHETASE AND PROTEIN HAVING DEACETYL-CEFALOSPORIN-C-SYNTHETASE ACTIVITY, HOST CELLS CAPABLE OF EXPRESSING PROTEIN, AS WELL AS CEFALOSPORIN
The present invention relates to DNA compounds and expression vectors that encode deacetoxycephalosporin C synthetase (DAOCS) and deacetylcephalosporin C synthetase (DACS) activities. The compounds can be used to construct recombinant DNA expression vectors for a wide variety of host cells, including E. coli, Penicillium, and Cephalosporium. The invention also relates to the regulatory elements of the Cephalosporium acremonium DACS/DAOCS gene. A method for selecting Penicillium transformants, including those able to synthesize cephalosporin antibiotic due to the presence of the DACS/DAOCS-encoding DNA, is also provided. This transformation system utilizes an acetamidase gene for purposes of selecting transformants.
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