ASSAY FOR 1,25-DIHYDROXY VITAMIN D IN A BIOLOGICAL SAMPLE CONTAINING VITAMIN D TRANSPORT PROTEIN

摘要

An assay for 1,25-dihydroxy vitamin D is disclosed. One aspect of the invention involves adding pig receptor protein, radiolabeled, 1,25-dihydroxy vitamin D and biotinylated antibody capable of binding to the receptor to untreated blood serum. In performing a competitive binding assay, vitamin D transport protein, DBP, acts as a screen to minimize interference from related metabolites. A kit and an assay are disclosed.