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method for measuring antigen or antikoerpern in biological fluessigkeiten using ligand labelled antigen or ligand selected antikoerpern.
method for measuring antigen or antikoerpern in biological fluessigkeiten using ligand labelled antigen or ligand selected antikoerpern.
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机译:用配体标记的抗原或配体选择的抗库珀菌素测定生物氟西汀中抗原或库珀菌素的方法。
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摘要
A method for measuring the level of circulating antigen (Ag1), antibody (Ab1) or hapten (H) in a liquid sample, which comprises forming in a liquid phase reaction a soluble complex wherein said antigen (Ag1) antibody (Ab1) or hapten (H) is linked through, respectively, a specific antibody (Ab), antigen (Ag) or antihapten (Anti-H), to a matrix which is soluble in the liquid phase and carries a ligand (X), subsequently forming an insolubilised complex comprising a solid support linked to the ligand (X) of said soluble complex through an anti-ligand (Y), the insolubilised complex carrying a label (Z) linked to the antigen (Ag1) through an anti-antigen (Anti-Ag1), to the antibody (Ab1) through an anti-antibody (Anti-Ab1) or to the hapten (H), washing the insolubilised complex, and checking the washed insolubilised complex for the presence of the label (Z) to provide a measure of the level of said antigen (Ag1), antibody (Ab1) or hapten (H) in said sample.
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