PROCESS AND REAGENT FOR THE SPECIFIC DETERMINATION OF FRUCTOSAMINE

摘要

ABSTRACTProcess and reagent for the specific determination offructosamineThe present invention provides a process for thespecific determination of the serum fructosamine contentin blood or samples derived from blood by reaction withan appropriate colour reagent and measurement of thecolour change thereby brought about, wherein, beforethe colour reaction, non-specific reducing-acting and/orturbidity-causing sample components are removed atapproximately neutral pH value, subsequently the pH isadjusted to a value of from 10 to 12 and the colourreagent is added thereto.The present invention also provides a reagentmixture for the specific determination of the serumfructosamine content in blood or samples derived fromblood, wherein it comprises a reagent for the removalof non-specific reducing-acting and/or turbidity-causing sample components, a rebuffering reagent witha buffer which has a pH value in the range of from 10.5to 12.5 and a colour reagent for the detection offructosamine.