首页>
外国专利>
AGGLUTINATION IMMUNOASSAY AND KIT FOR DETERMINATION OF A MULTIVALENT IMMUNE SPECIES USING A BUFFERED SALT WASH SOLUTION
AGGLUTINATION IMMUNOASSAY AND KIT FOR DETERMINATION OF A MULTIVALENT IMMUNE SPECIES USING A BUFFERED SALT WASH SOLUTION
展开▼
机译:凝聚免疫分析和试剂盒,用于缓冲盐洗液测定多价免疫物种
展开▼
页面导航
摘要
著录项
相似文献
摘要
AGGLUTINATION IMMUNOASSAY AND KIT FOR DETERMINATIONOF A MULTIVALENT IMMUNE SPECIES USINGBUFFERED SALT WASH SOLUTIONAbstract of the DisclosureA test kit is used in an agglutinationimmunoassay to determine a multivalent immunespecies, such as Streptococcus A antigen, in a bio-logical sample. The method includes contacting anaqueous solution of the species with an agglutinationindicator reagent having receptor molecules reactiuewith the species to form an agglutinate of the reac-tion product of species and receptor. These receptormnlecules are bound to polymeric particles whichcontain tracer molecules. The resulting agglutinateis captured on a microporous membrane which has anaverage pore size which is at least five timesgreater than the average diameter of the polymericparticles. Unagglutinated residual materials arewashed through the membrane using a wash solution.This solution has a pH of from about 5 to about 10and an ionic strength of at least about 0.25. Traceris then determined either in the agglutinate or inthe residual materials. The test kit includes theagglutination indicator reagent, the wash solution,and optionally an extraction composition.
展开▼