DNA PROBES AND PRIMERS FOR DETECTION OF B. BURGDORFERI USING THE POLYMERASE CHAIN REACTIONS

摘要

2064582 9119814 PCTABS00008 Nucleic acid primers of complementary sequence to portions of the gene encoding a semiconserved region of B. burgdorferi flagellin are utilized to amplify target sequences of nucleic acids extracted from infective sites of Lyme borreliosis, which are then probed with a nucleic acid tracer conjugated to signal-generating molecules for highly specific detection of B. burgdorferi.