Indirect colorimetric detection of an analyte in a sample using ratio of light signals

摘要

The method of the invention employs an enzyme immunoassay for measuring the concentration of an analyte in a sample by indirect colorimetric detection. An incident light beam or a plurality of wavelengths is directed into a liquid solution containing an analyte of interest; the solution being capable of attentuating the amount of light at a first wavelength received from this solution as a function of the increasing concentration of the analyte present. A light signal from the solution at the first wavelength is detected, and light at a second wavelength, at which substantially no attenuation of light signal occurs as the concentration of the analyte increases, is also detected. The ratio of the two respective wavelengths is formed and that ratio is compared with ratios of known amounts of the analyte to determine the amount of tha analyte in the sample.