METHOD OF SEPARATION AND CONCENTRATION OF STEROL AND TOCOPHEROL FROM DISTILLATED SOYBEAN POWDER

摘要

A process for separating and concentrating sterol and tocopherol from deodorized distillate of soybean oil comprises (A) adding mixed solvent of n-hexane and 50 % (V/V) methanol to soybean oil distillate, (B) cooling the mixture to 5 deg.C -10 deg.C to separate sterol first, (C) methyl esterifying the mixture from which sterol is removed, (D) recirculating esterified product in a molecular distiller at 100-130 deg.C and 10-2 - 10-4 torr to remove fatty acid methyl ester, and (D) recirculating again it at 170-200 deg.C and 10-2 - 10-4 torr to separate and concentrate tocopherol. The mixing ratio of n-hexane to methanol is 10:1, and addition amount of mixed solvent is 2 or 3 times the amount of soybean oil distillate.