METHOD FOR DETERMINATION OF SUPEROXIDE DISMUTASE ACTIVITY IN BLOOD

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to medicine and can be used u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 activity u0441u0443u043fu0435u0440u043eu043au0441u0438u0434u0434u0438u0441u043cu0443u0442u0430u0437u044b in u0445u0440u043eu0432u0438. the purpose of u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 -- improving the sensitivity and accuracy of u0438u0437u043cu0435u0440u0435u043du0438u00a0 activity enzyme u0441u0443u043fu0435u0440u043eu043au0441u0438u0434u0434u0438u0441u043cu0443u0442u0430u0437u044b (ods) in human blood. u0434u043bu00a0 this from the blood of patients receiving extracts / containing ods.u0438u0437u043cu0435u0440u00a0u044eu0442 u043bu044eu0446u0438u0433u0435u043du0438u043d - dependent u0445u0435u043cu0438u043bu044e - u043cu0438u043du0435u0441u0446u0435u043du0446u0438u044e reflecting the intensity of u043eu0431u0440u0430u0437u043eu0432u0430u043du0438u00a0 u0441u0443u043fu0435u0440u043eu043au0441u0438u0434u043du044bu0445 radicals in u0430u0443u0442u043eu043eu043au0438u0441u043bu0435u043du0438u0438 u043au0432u0435u0440u0446u0435u0442u0438u043du0430 and u0438u043du0433u0438u0431u0438u0440u043eu0432u0430 - no e of the u0441u0432u0435u0447u0435u043du0438u00a0 ods - containing extracts of blood. on the u043au0430u043bu0438u0431u0440u043eu0432u043eu0447u043du043eu043cu0443 schedule, centering u0434u043bu00a0 commercial preparations of enzyme, u043eu043fu0440u0435u0434u0435u043bu00a0u044eu0442 of ods in the blood samples. 1, table 2).water u0441u043fu0438u0440u0442u043eu0432u0430u00a0 contains u0441u0443u043fu0435u0440u043eu043au0441u0438u0434u0434u0438u0441 - u043cu0443u0442u0430u0437u0443 (ods), in the form of a thin film u0438u043du0442u0435u0440u0444u0430u0437u0430 dark red colors include hemoglobin and u043du0438u0436u043du00a0u00a0 - u043fu0440u043eu0437u0440u0430u0447u043du0430u00a0, u0445u043bu043eu0440u043eu0444u043eu0440u043cu043du0430u00a0, ods to contains. carefully selected from the upper part of the u0435u0443u043fu0435u0440u043du0430u0442u0430u043du0442u0430 and u043fu0435u0440u0435u043du043eu0441u00a0u0442 in a test tube, u0440u0430u0437u0432u043eu0434u00a0u0442 50 times 20 mm u0444u043eu0441u0444u0430u0442u043du043eu043c buffer (ph 7.8) and further use u0434u043bu00a0 anal isa.separately, then cook the standard solutions of commercial ods (Beringher) with the source u043au043eu043du0446u0435u043du0442u0440u0430u0446u0438u00a0u043cu0438 800, 400. 200, 100. 50, 25, and 12.5 ng / ml. ii. the u0445u043b - analysis. in the ditch, shall be u0434u043bu00a0 u0438u0437u043cu0435u0440u0435u043du0438u00a0 u0445u043b u0434u043eu0431u0430u0432u043bu00a0u044eu0442 700 mkl environment containing 20 mm phosphate buffer (u043au043d2u044004 ferropur (nah u044004 - 100 microns u044du0434u0442u0430 (u044du0442u0438u043bu0435u043du0434u0438u0430u043cu0438u043du0442u0435u0442u0440u0430u0430u0446u0435u0442u0430u0442 na), 40 to 60 microns u043bu044eu0446u0438u0433u0435u043du0438u043du0430 (sigma), ph 9.6.u0440u0435u0433u0438u0441u0442u0440u0430u0446u0438u00a0 u0445u043b was in u043bu044eu043cu0438u043du043eu043cu0435u0442u0440u0435 1KB 1251 (u0448u0432u0435u0446u0438u00a0) in continuous mode with temperature 27 & deg; c and u043fu043eu0441u0442u043eu00a0u043du043du043eu043c displacement of off the road. over 2 million (about 00