Determn. of lactose repressor over-producing microorganisms - using a phage with a marker gene contg. C-terminal with a lactose operator and promoter which forms an anti-sense RNA of the marker

摘要

The microorganism are transfected with a bacteriophage carrying a detectable marker joined at its C-terminus with a lactose-promoter/operator which is transcribed as an anti-sense RNA of the marker. The expression prods. are detected. Pref. the phage is Lambda or its derivs., and the marker is the xylE-gene of Pseudomonas (P.) putida or the Glowing gene of Vibrio fischeri. USE/ADVANTAGE - Described is a simple detection method for genetically engineered microorganisms which carry a modified lac repressor gene and which, for example, may have escaped from a laboratory environmentntn