Affinity chromatography for heterogeneous immunoassay or preparative purificn. - using lyotropic liq. crystalline stationary phase, and organic solvent or reverse micellar mobile phase

摘要

In an affinity chromatography process using an organic solvent mobile phase, a lyotropic liq. crystalline (LLC) system (or a microemulsion gel, i.e. an LLC/microemulsion gel, or its mixt. with an inert carrier) is used to immobilise binding-active substances (A). This system coexists with an organic solvent or reversed-micellar phase in which the binding partner is dissolved or solubilised. Quantification or further processing is carried out in this mobile phase, or after release from the stationary phase, or in the stationary phase (after this has been converted to liq. form by addn. of other substances, application of pressure and/or by heating). USE/ADVANTAGE - The method is used for (1) performing heterogeneous immunoassays (esp. ELISA) or other binding tests or (2) preparative purification. Suitable (A) are antibodies, antigens, ligands, receptors or other biological binding partners and their conjugates, esp. those of poor water solubility. Analytes are selectively sepd. from the solvent phase with high binding capacity, simply and reproducibly, then converted back to a highly conc. mobile phase without an elution stage (so the detection limit is improved).