METHOD FOR MANUFACTURING VON WILLEBRAND FACTOR HAVING VERY HIGH PURITY, HAVING A MAJOR PORTION OF ANTIHEMOPHILIC FACTOR (FVIIIC), AND VON WILLEBRAND FACTOR THUS OBTAINED, AND A PHARMACEUTICAL COMPOSITION CONTAINING SAME

摘要

The invention relates to a process for the manufacture of von Willebrand factor. BR/ This process comprises the selective adsorption of Factor VIIIc on the gel of a first ion exchange chromatography, the von Willebrand Factor being contained in the non-retained fraction, and is characterized in that the non-retained fraction is treated so as to selectively adsorb the von Willebrand Factor on the gel of a second chromatography column which is then desorbed by being obtained with a very high purity. BR/ The invention makes it possible to obtain a very high purity von Willebrand factor, largely free of antihemophilic factor VIIIc, inactivators such as Tween and TNBP, as well as major plasma contaminants. / P