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Immobilization of biologically active material in capsules prepared from a water-soluble polymer and chitosan acetate
Immobilization of biologically active material in capsules prepared from a water-soluble polymer and chitosan acetate
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机译:将生物活性物质固定在由水溶性聚合物和壳聚糖乙酸酯制成的胶囊中
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摘要
Biologically-active material, particularly hybridoma cells, is immobilized for mass culture in bioreactors to produce biological products, particularly monoclonal antibodies, by ionically-interacting polycationic groups on chitosan with polyanionic groups on a polyanionic water-soluble polymer. Preferably, the chitosan is chitosan acetate and the polyanionic polymer is sodium alginate. In one embodiment, droplets of a suspension of the biologically-active material and water-soluble polymer are gelled by forming a calcium salt of the water-soluble polymer to form temporary capsules, and a sequestering agent for the calcium ions is added to effect ionotropic gelation of the water-soluble polymer and chitosan to form a semi-permeable membrane around each temporary capsule. In another embodiment, porous beads are formed by exposing droplets of a suspension of the biologically active material and water-soluble polymer to an aqueous solution of chitosan and multiple cations. In a further embodiment, chitosan is added to a suspension of the biologically active material and water-soluble polymer and the resulting suspension is extruded into a solution of multi-valent anions to form a fibrous mass.
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