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Host expressing NgoAIII restriction endonuclease and modification methylase from neisseria
Host expressing NgoAIII restriction endonuclease and modification methylase from neisseria
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机译:表达来自奈瑟氏球菌的NgoAIII限制性核酸内切酶和修饰甲基化酶的宿主
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摘要
The present invention is directed to recombinant hosts which contain and express various Type II restriction endonuclease and/or modification methylase genes. In particular, the present invention is concerned with the cloned restriction endonucleases, NgoAIII and NgoAI, which recognize and cleave within or near the double-stranded DNA sequence, 5' CCGCGG 3' and 5' PuGCGCPy 3', respectively. Also provided in this invention are cloned modification methylase genes corresponding to said restriction endonucleases. This invention is further concerned with a cloned modification methylase, M.NgoAII. One source of these enzymes is Neisseria gonorrhoeae, although other microorganisms may be used to isolate the restriction endonuclease isoschizomers and modification methylase isoschizomers of this invention.
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