TANK FOR SAPONIFICATION OF SAMPLE FOR ANALYSIS OF FAT-SOLUBLE VITAMINS AND RAPID SAPONIFICATION-EXTRACTION OF SAMPLE FOR ANALYSIS OF FAT-SOLUBLE VITAMINS USING THE SAME TANK

摘要

PURPOSE:To carry out rapid saponification-extraction of the subject sample useful as a food, a medicine, etc., by using a specified tank in which the reagent-accommodating unit is excellent in transparency, chemical resistance and mechanical strength due to its constituting material, its structure, etc. CONSTITUTION:An objective extraction is carried out by using a tank having a transparent sample-accommodating unit excellent in chemical resistance and mechanical strength enough to resist a centrifugal force of =1G in a state where =10g liquid is accommodated due to its constituting material (preferably borosilicate glass) and its structure and a mouth part coverable with a chemical-resistant material for tightly sealing (composed of cover, etc., made of silicone resin, etc., and having screw type structure, etc.) the tank so that the tank may endure an inner pressure of =0.5kg/cm2, adding 1-2ml 90wt.% KOH to 0.5-2.0g sample, further adding 5ml 10wt.% pyrogallol-containing ethanol thereto, then tightly sealing the tank, carrying out saponification in a heating bath, etc., subsequently cooling it, carrying out centrifugation, further adding 3-5ml benzene thereto, then tightly sealing the tank, adding aqueous saturated edible salt solution, etc., under shaking, etc., till the whole volume becomes 10ml, separating a benzene layer by centrifugation, subsequently adding aqueous saturated edible salt solution, etc., again till the whole volume becomes 10ml, separating a benzene layer by centrifugation and then sampling a prescribed amount of the resultant separated benzene layer.