PRODUCTION OF HUMAN PROLYL HYDROXYLASE BETA-SUBUNIT AND DETERMINATION OF HUMAN PROLYL HYDROXYLASE USING SAID BETA-SUBUNIT AS STANDARD

摘要

PURPOSE:To carry out determination of an immunoreactive human prolyl hydroxylase (hPH) using a beta-subunit as a standard, by isolating the beta-subunit from a digestion product prepared by reacting the hPH with collagenase. CONSTITUTION:Human prolyl hydroxylase (hPH) is reacted with collagenase to provide a digestion product, which is then treated with an affinity column containing a linked monoclonal antibody to prolyl hydroxylase to separate and collect hPH beta-subunit exhibiting a single band in electrophoresis. The resultant hPH beta-subunit is used as a standard to determine immunoreactive hPH by a sandwich enzyme immunoassay.