CATECHOL AMINE MEASURING REAGENT AND QUANTIFYING METHOD FOR CATECHOL AMINE USING IT

摘要

PURPOSE: To quantify catechol amine simply, at a low cost, and with high sensitivity by using an aryl aminomethyl derivative given by a specific expres sion as a fluorescent derivative reagent. ;CONSTITUTION: An aryl aminomethyl, derivative as given by formula I as a peculiar fluorescent derivative reagent for catechol amine (where, Ar is phenyl group and L is eliminated group) is used as a fluorescent derivative reagent, and under co-existence with catalyst, oxidator, and reaction accelerator, reactions are made with catechol amine at pH4-9. The catalyst may preferably be ammonium molybdene, oxidator be KIO₄, and reaction accelerator be mathanol or ethanol. Pyrrolobenzoxazole derivative of fluorescence generating substrate as given by formula II (where, Ar is aryl radical, R₁ and R₂ are hydrogen atom, hydroxyl radical, or methyl radical either identical or different) which is fluorescence reaction resultant of aryl aminomethyl derivative and catechol amine, is a very strong fluorescence substrate working stably.;COPYRIGHT: (C)1993,JPO&Japio