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GENE CAPABLE OF SUPPRESSING PRODUCTION OF HYDROGEN SULFIDE WITH YEAST AND YEAST FOR BREWING CONTAINING THE SAME GENE TRANSDUCED THEREINTO
GENE CAPABLE OF SUPPRESSING PRODUCTION OF HYDROGEN SULFIDE WITH YEAST AND YEAST FOR BREWING CONTAINING THE SAME GENE TRANSDUCED THEREINTO
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机译:能够抑制酵母和酵母产生硫化氢的基因以及用于发酵包含相同基因的酵母的酵母
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摘要
PURPOSE:To suppress the ability to produce hydrogen sulfide and enable obtaining of beer in a short time by transducing a gene (NHS5) capable of exhibiting a prescribed base sequence suppressing the production of the hydrogen sulfide with a yeast. CONSTITUTION:A plasmid (pHS5), capable of suppressing H2S and having a well-known gene capable of suppressing H2S is subcloned to prepare a gene (NHS5) that is a protein having an amino acid sequence composed of a sequence of bases, etc., expressed by the formula in which the interval between prescribed positions is coded in the base sequence. The resultant NHS5 is then treated with a restriction enzyme to afford a plasmid (e.g. YCpAG-5SS), which is subsequently transduced into a yeast of the genus Saccharomyces for brewing to provide a transformant. The obtained transformant is then inoculated into a wort containing a hop added thereto and fermented to reduce the amount of the produced hydrogen sulfide from the parent strain by, 20-40%.
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