SUPRAVITAL ANALYSIS METHOD OF BLOOD THROUGH DIFFERENT-COLOR STAINING AND FLUORESCENT LUMINESCENCE

摘要

Lengthy cytochemical procedures for differentiation, identification, enumeration and diagnostic study of leukocytes in human blood have been advanced and shortened by a rapid method of optical differentiation of the five individual white blood cell species by selective use of basic quaternary metachromatic dye staining of supravital blood at controlled temperature. Presently a manual method, the improvement provides essential groundwork for improved accuracy in automated differential leukocyte counting.