The composition, analogo of oligonucleotideo, oligonucleotideo sequenced joint, process for the preparation of nucleosideos 2 '- replaced, structure to modulate the activity of RNA.Processes for the production of a modular protein by a body, to treat an animal, to detect the presence or absence of the RNA.Oligonucleotideo or analogo of analogo of oligonucleotideo resistant to nuclease and process for treating a body having a disease

摘要

Compositions and methods for modulating the activity of RNA and DNA are disclosed. In accordance with preferred embodiments, antisense compositions are prepared comprising targeting and reactive portions. Reactive portions which act, alternatively, through phosphorodiester bond cleavage, through backbone sugar bond cleavage or through base modification are preferably employed. Groups which improve the pharmacodynamic and pharmacokinetic properties of the oligonucleotides are also useful in accordance with certain embodiments of this invention. Delivery of the reactive or non-reactive functionalities into the minor groove formed by the hybridization of the composition with the target RNA is also preferably accomplished. Therapeutics, diagnostics and research methods are also disclosed. Synthetic nucleosides and nucleoside fragments are also provided useful for elaboration of oligonucleotides and oligonucleotide analogs for such purposes.